Biochem/physiol Actions

Reversible: yes

Product does not compete with ATP.

Primary TargetPS3

Cell permeable: yes

General description

A cell-permeable chemical inhibitor of p53. Reversibly inhibits p53-dependent transactivation of p53-responsive genes and reversibly blocks p53-mediated apoptosis. Inhibits p53-dependent growth arrest of human diploid fibroblasts in response to DNA damage but has no effect on p53-deficient fibroblasts. Protects normal tissues from the deleterious side effects of chemotherapy. Has been reported to protect neurons against β-amyloid peptide and glutamate-induced apoptosis.

A reversible inhibitor of p53-dependent transactivation of p53-responsive genes and reversibly blocks p53-mediated apoptosis. Inhibits p53-dependent growth arrest of human diploid fibroblasts in response to DNA damage but has no effect on p53-deficient fibroblasts. Protects mice from lethal genotoxic stress associated with anticancer treatment without promoting tumor formation. Has been reported to protect neurons against β-amyloid and glutamate-induced apoptosis.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Murphy, P.J.M., et al. 2004. J. Biol. Chem.279, 30195.Culmsee, C., et al. 2001. J. Neurochem.77, 220.Komarova, E.A., and Gudkov, A.V. 2000. Biochemistry (Mosc.) 65, 41.Stadler, P., et al. 2000. Strahlenther Onkol. 176, 98.Ferber, D. 1999. Science 285, 1651.Jacquemin-Sablon, A. 1999. Bull. Cancer86, 894.Komarov, P.G., et al. 1999. Science 285, 1733.

Packaging

Packaged under inert gas

5, 10 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot into cold tubes and freeze (-20°C). Stock solutions in DMSO are stable for 3 months at -20°C. Unstable in aqueous solutions.

Warning

Toxicity: Standard Handling (A)